An idiotypic-anti-idiotypic competitive immunoassay for quantitation of okadaic acid
1992; Elsevier BV; Volume: 30; Issue: 11 Linguagem: Inglês
10.1016/0041-0101(92)90519-b
ISSN1879-3150
AutoresWilliam S. Shestowsky, Michael A. Quilliam, Hanna Sikorska,
Tópico(s)Hemoglobin structure and function
ResumoA competitive indirect enzyme-linked immunosorbent assay for the measurement of okadaic acid, a marine toxin, was developed. The assay uses a murine monoclonal anti-idiotypic antibody bearing an internal image of okadaic acid epitope to capture an anti-okadaic acid monoclonal antibody in the presence of free okadaic acid. Bound anti-okadaic acid antibody is detected with peroxidase-conjugated anti-mouse immunoglobulin antiserum. If present, free toxin will lessen the amount of anti-okadaic acid antibody binding to its corresponding anti-idiotypic antibody in a dose-dependent manner that can be quantified from the standard curve. The assay permits reliable measurement of okadaic acid in the 9–81 ng/ml range. The intra- and interassay coefficients of variation in the measurement of OA in the toxin spiked mussel samples averaged 9% and 12%, respectively. The assay is rapid, accurate, reproducible and relatively simple to perform. It may be of potential use to laboratories involved in monitoring the toxin levels in plankton, seafood or sponges.
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