Portal de Periódicos da CAPES

Artemisinin production by Artemisia annua L.-transformed organ cultures

1996; Elsevier BV; Volume: 18; Issue: 7 Linguagem: Inglês

10.1016/0141-0229(95)00216-2

1879-0909

Norma Paniego, Ana María Giulietti,

Herbal Medicine Research Studies

Transformed shoot and root cultures of Artemisia annua L. were established by infection with Agrobacterium tumefaciens strain T37 and A. rhizogenes LBA 9402. These cultures were able to grow in a hormone-free medium and produce artemisinin during prolonged subcultures. Several clones of transformed shoots were obtained and the average artemisinin content found in them was approximately 0.018 ± 0.004% dry weight (DW). Changes in Ca2+, Mg2+, and PO43− concentration of the RT vitamin complex medium (MSRT) did not induce an increase in artemisinin production. The addition of plant growth regulators such as GA3 improved the artemisinin production of 300–400% of the control value. Transformed root cultures showed a strong morphological instability and accumulated low levels of artemisinin (0–0.01% DW).