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Bildung von sekundären wandstrukturen in frei suspendierten einzelzellen von pelargonien-kallus in Flüssigkultur

1973; Elsevier BV; Volume: 70; Issue: 3 Linguagem: Inglês

10.1016/s0044-328x(73)80084-4

0044-328X

G. Reuther, Peter Werckmeister,

Plant Molecular Biology Research

Summary Expiants of shoot apices from varieties of Pelargonium zonale and of Pelargonium pel-tatum × Pelargonium zonale were cultured on a solid basal medium according to Murashige-Skoog supplemented with 15 % cocosmilk. The effect of NAA and 2,4-D on callus growth and differentiation was compared at a concentration of 1 ppm. In liquid medium the mass of cells in juvenile cultures were freely suspended parenchymatic single cells of abnormal size (giant cells). The diameter of cells in callus clumps was strinkingly smaller. After a shaking period of 3 month in single cells tracheary elements were formed. A great number of globular and cylindrical pitted cells and some with reticulated thickenings could be identified. This type of tracheidal cell differed from tracheids differentiated in the center of nodules in callus tissue. It is concluded that kinetin like substances favour polyploidy and the differentiation by formations of secondary wall elements. Under the described conditions callus cells from shoot apices of Pelargonium undergo two different ways of organization: 1. Formation of giant parenchymatous and tracheidal single cells without any connection with greater tissue units, 2. formation of callus clumps with small cells and tracheids in the center.