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Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device

2014; Elsevier BV; Volume: 119; Issue: 4 Linguagem: Inglês

10.1016/j.jbiosc.2014.09.018

1389-1723

Hitoyoshi Yamashita, Masamune Morita, Haruka Sugiura, Kei Fujiwara, Hiroaki Onoe, Masahiro Takinoue,

Electrowetting and Microfluidic Technologies

We report an easy-to-use generation method of biologically compatible monodisperse water-in-oil microdroplets using a glass-capillary-based microfluidic device in a tabletop mini-centrifuge. This device does not require complicated microfabrication; furthermore, only a small sample volume is required in experiments. Therefore, we believe that this method will assist biochemical and cell-biological experiments.