On the rapid deprotection of synthetic oligonucleotides and analogs
1994; Oxford University Press; Volume: 22; Issue: 4 Linguagem: Inglês
10.1093/nar/22.4.639
ISSN1362-4962
AutoresNikolai N. Polushin, A. M. Morocho, Ban-chin Chen, Jack S. Cohen,
Tópico(s)Chemical Analysis and Environmental Impact
ResumoThe efficiency of ollgodeoxynucleotlde deprotection is greatly enhanced using a combination of: (a) ethanolamlne, and especially a mixture of hydrazine, ethanolamine and methanol, in place of the usual aqueous ammonia; (b) tert-butylphenoxyacetyl amino protecting groups, and (c) oxalyl link between the first nucleotlde and the polymeric support. The extent of base modification, particularly of C, is shown to be extremely low, and the quality of deprotected oligonucleotides is as high as In the case of ammonia deprotection. This method Is also shown to be applicable to the preparation of phosphorothloate and methylphosphonate oligodeoxynucleotldes and oligoribonucleotldes.
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