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Binding of integration host factor (IHF) to the ilvGp1 promoter of the ilvGMEDA operon of Escherichia coli K12

1988; Oxford University Press; Volume: 16; Issue: 13 Linguagem: Inglês

10.1093/nar/16.13.5973

1362-4962

R. F. Pereira, Manuel Ortuño, Robert P. Lawther,

Antibiotic Resistance in Bacteria

Crude extracts of Escherichia coli K-12 were found to bind DNA restriction fragments containing ilvGp1 Our analysis using a series of restriction fragments and a BamHI linker mutation indicate that a factor binds to ilvGp1 or adjacent to it. Analysis with mutant strains of E. coli K-12 and purified IHF indicate that IHF binds to ilvGp1 Furthermore, both analysis in vivo and in vitro indicate that IHF precludes transcription from ilvGp1.