Detección y expresión de una proteína de unión a calmodulina en Plasmodium falciparum
2001; National Institute of Health; Volume: 21; Issue: 1 Linguagem: Inglês
10.7705/biomedica.v21i1.1086
ISSN2590-7379
AutoresÁngela Patricia Guerra, Víctor Vera, Jacqueline Chaparro‐Olaya, Moisés Wasserman,
Tópico(s)Venomous Animal Envenomation and Studies
ResumoMonoclonal antibodies were produced against affinity chromatography purified calmodulin binding proteins (CaMBP) of Plasmodium falciparum. Using one of them (1G3) in a Western blot assay, a 30 kDa calmodulin binding protein was detected in extracts of erythrocytes infected with Plasmodium falciparum. This protein was not detected in extracts of non infected erythrocytes. Additionally, the antibody presented a cross reaction with two bands 260 and 240 kDa, that reacted also with anti-spectrin policlonal antibody, suggesting that a similar epitope exists in the 30 kDa CaMBP and in spectrin. A study of expression of the 30 kDa CaMBP during the erythrocytic cell cycle showed a stage-specific expression pattern, detecting it only in the late stages of the parasite and not in the younger ones.
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