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Rapid Identification of Positive Blood Cultures by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Using Prewarmed Agar Plates

2014; American Society for Microbiology; Volume: 52; Issue: 12 Linguagem: Inglês

10.1128/jcm.01788-14

1098-660X

Micah M. Bhatti, Sue Boonlayangoor, Kathleen G. Beavis, Vera Tešić,

Diphtheria, Corynebacterium, and Tetanus

ABSTRACT This study describes an inexpensive and straightforward method for identifying bacteria by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) directly from positive blood cultures using prewarmed agar plates. Different inoculation methods and incubation times were evaluated to determine the optimal conditions. The two methods using pelleted material from positive culture bottles performed best. In particular, the pellet streak method correctly identified 94% of the Gram negatives following 4 h of incubation and 98% of the Gram positives following 6 h of incubation.