Structure, Microtubule Interactions, and Paired Helical Filament Aggregation by Tau Mutants of Frontotemporal Dementias
2000; American Chemical Society; Volume: 39; Issue: 38 Linguagem: Inglês
10.1021/bi000850r
ISSN1943-295X
AutoresStefan Barghorn, Qingyi Zheng‐Fischhöfer, Michael Ackmann, Jacek Biernat, Martin von Bergen�, Eckhard Mandelkow�, Eckhard Mandelkow�,
Tópico(s)Prion Diseases and Protein Misfolding
ResumoWe have studied biochemical and structural parameters of several missense and deletion mutants of tau protein (G272V, N279K, ΔK280, P301L, V337M, R406W) found in frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17). The mutant proteins were expressed on the basis of both full-length tau (htau40) and constructs derived from the repeat domain. They were analyzed with respect to the capacity to enhance microtubule assembly, binding of tau to microtubules, secondary structure content, and aggregation into Alzheimer-like paired helical or straight filaments. We find that the mutations cause a moderate decrease in microtubule interactions and stabilization, and they show no gross structural changes compared with the natively unfolded conformation of the wild-type protein, but the aggregation into PHFs is strongly enhanced, particularly for the mutants ΔK280 and P301L. This gain of pathological aggregation would be consistent with the autosomal dominant nature of the disease.
Referência(s)