Construction by homologous recombination and phenotypic characterization of a DNA polymerase domain polA mutant of Mycobacterium smegmatis
1996; Elsevier BV; Volume: 178; Issue: 1-2 Linguagem: Inglês
10.1016/0378-1119(96)00350-2
ISSN1879-0038
AutoresBhavna G. Gordhan, Susan J. Andersen, Anna Meyer, Valerie Mizrahi,
Tópico(s)Antibiotic Resistance in Bacteria
ResumoGene replacement was achieved by homologous recombination in Mycobacterium smegmatis (Ms) using a cloned segment of the polA gene (encoding the DNA polymerase I) disrupted within the region encoding the C-terminal DNA polymerase domain by a kanamycin-resistance marker. The Ms polA755:aph mutant thus generated displayed a phenotype of hypersensitivity to DNA damage induced by UV irradiation and by hydrogen peroxide challenge.
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