Crystallization and preliminary X-ray crystallographic analysis of squalene-hopene cyclase from Alicyclobacillus acidocaldarius
1997; Wiley; Volume: 6; Issue: 3 Linguagem: Inglês
10.1002/pro.5560060322
ISSN1469-896X
AutoresK. Ulrich Wendt, Alexander Lenhart, Georg E. Schulz, Corinna Feil, Karl Poralla,
Tópico(s)Photosynthetic Processes and Mechanisms
ResumoThe membrane-associated protein squalene-hopene cyclase from Alicyclobacillus acidocaldarius was overexpressed in Escherichia coli and purified by ion exchange and gel permeation chromatography. Crystals of three interrelated forms were grown by vapor diffusion under identical conditions. The crystals diffract to about 2.3 A resolution, but they are unstable in the X-ray beam. An interpretable heavy-atom derivative was obtained.
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