Portal de Periódicos da CAPES

Photoisomerization of the green fluorescence protein chromophore and the meta- and para-amino analogues

2008; Royal Society of Chemistry; Issue: 11 Linguagem: Inglês

10.1039/b717714c

1364-548X

Jye‐Shane Yang, Guan‐Jhih Huang, Yi‐Hung Liu, Shie‐Ming Peng,

Photochemistry and Electron Transfer Studies

The Z → Ephotoisomerization and fluorescence quantum yields for the wild-type green fluorescence protein (GFP) chromophore (p-HBDI) and its meta- and para-amino analogues (m-ABDI and p-ABDI) in aprotic solvents (hexane, THF, and acetonitrile) and protic solvents (methanol and 10–20% H2O in THF) are reported. The dramatic decrease in the quantum yields on going from aprotic to protic solvents indicates the important role of solvent–solute hydrogen bonding in the nonradiative decay pathways. The enhanced fluorescence of m-ABDI is also discussed.