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COMPARISON OF CULTURED HUMAN MONONUCLEAR CELLS, LIMULUS AMEBOCYTE LYSATE and RABBITS IN THE DETECTION OF PYROGENS

2008; Wiley; Volume: 15; Issue: 6 Linguagem: Inglês

10.1111/j.1365-2710.1990.tb00407.x

1365-2710

Erik W. Hansen, Jens D. Christensen,

Animal Genetics and Reproduction

Isolated human mononuclear cells exposed to either lipopolysaccharide or Staphylococcus aureus secreted interleukin-1 like material. The secretion was concentration dependent. The minimal detectable level in the test solution of lipopolysaccharide and Staphylococcus aureus was 200 pg/ml and 105 cells/ml respectively. The sensitivity and specificity of the Limulus Amebocyte Lysate test, the rabbit pyrogen test and the monocyte test are compared. The monocyte test is proposed as an alternative in-vitro test to the rabbit pyrogen test.