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Role of lipopolysaccharide in the induction of type I interferon-dependent cross-priming and IL-10 production in mice by meningococcal outer membrane vesicles

2009; Elsevier BV; Volume: 27; Issue: 13 Linguagem: Inglês

10.1016/j.vaccine.2009.01.109

1873-2518

Vanessa Durand, Joanne MacKenzie, Joel de León, Circe Mesa, Valérie Quesniaux, María Montoya, Agnès Le Bon, Simon Y. C. Wong,

Pneumonia and Respiratory Infections

We investigated the contribution of lipopolysaccharide (LPS) to adjuvant properties of native outer membrane vesicles (NOMV), a vaccine candidate for meningococcal B disease. NOMV induce the maturation of and cytokine production by murine bone marrow-derived dendritic cells through both toll-like receptors (TLR) 2 and 4 which are mostly dependent on the signalling adaptor MyD88. NOMV are also able to induce B cell proliferation in splenocytes from LPS-hyporesponsive mice. However, induction of IL-10 and type I interferon-dependent, antigen-specific and IFNγ-secreting CD8+ cytotoxic T lymphocyte responses in vivo by NOMV requires LPS. The importance of LPS in the induction of IL-10 and functional cross-priming has implications for NOMV-based vaccine and adjuvant development.