Portal de Periódicos da CAPES

Effect of ooplast activation on the development of nuclear transferred bovine embryos

1993; Elsevier BV; Volume: 39; Issue: 1 Linguagem: Inglês

10.1016/0093-691x(93)90103-c

1879-3231

Takahiro Kono, Y. Sotomoru, Fumihito Aono, T. Takahashi, Kikumi OGIHARA, Fumio SEKIZAWA, Touru Arai, Tsutomu Nakahara,

Sperm and Testicular Function

The aim of the work was to determine the susceptibility of non-cultured and cultured rabbit embryos at different stages of development to the adverse effects of vitrification. The experiment was carried out on non-cultured embryos at the 8- to 16-cell, morula and blastocyst stages, and on embryos collected at the 2-cell stage and cultured for 24, 48 or 72 h. The embryos were cultured in Menezo's B2 medium supplemented with 20% FCS (fetal calf serum), or were cocultured with oviductal epithelium cells. A mixture of 35% glycerol + 35% 1,2-propanediol in serum-supplemented, phosphatebuffered saline was used as a vitrification medium. The embryos were vitrified in glass ampoules or plastic straws. Dilution after rapid thawing was done in one step, in a 1 M sucrose solution.After vitrification of non-cultured embryos in glass ampoules, the survival rates of the 8- to 16-cell, morula and blastocyst stages were 55.9%, 88.4% and 83.1%, respectively.The survival rates of embryos derived from cultures of 2-cell embryos in Menezo's B2 medium for 24 h (8- to 16-cell stage), 48 h (morula stage) and 72 h (blastocyst stage) and then vitrified in glass ampoules were 46.7%, 62.4% and 36.2%, respectively. The percentage of live foetuses remaining 28 days after the transfer of 103 vitrified and 115 cultured and vitrified 8- to 16-cell embryos was 21.4% and 1.8%, respectively. After the transfer of 111 vitrified morulae and 112 cultured and vitrified morulae, the percentage of live foetuses 28 days later was 11.7% and 7.1%, respectively.After vitrification in plastic straws, the survival rates in vitro of non-cultured morulae, and of morulae resulting from the culture of 2-cell embryos for 48 h in Menezo's B2 medium and in the coculture system were 89.1%, 93.8% and 91.2%, respectively.