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S-Allyl Cysteine Inhibits Activation of Nuclear Factor Kappa B in Human T Cells

1997; Elsevier BV; Volume: 23; Issue: 2 Linguagem: Inglês

10.1016/s0891-5849(97)00006-3

1873-4596

Zhaohui Geng, Yongqi Rong, Benjamin H.S. Lau,

Fungal Plant Pathogen Control

Reactive oxygen species are involved in signal transduction pathways leading to nuclear factor kappa B (NF-κB) activation which has been implicated in the regulation of gene transcription. We recently reported that a garlic compound, S-allyl cysteine (SAC), protects bovine pulmonary artery endothelial cells from oxidant injury induced by hydrogen peroxide (H2O2). In this study we determined the effects of SAC on NF-κB activation in human T lymphocytes (Jurkat cells) induced by tumor necrosis factor α (TNF-α) and H2O2. Activated NF-κB in nuclear extracts was measured by an electrophoretic mobility shift assay using 32P-labeled probe. SAC consistently exhibited a dose-dependent inhibition of NF-κB activation induced by both TNF-α and H2O2. Supershift with specific antibodies to NF-κB subunits confirmed that the inducible retarded bands observed in the EMSA are p65-p50 heterodimer of the NF-κB/Rel protein. Our data suggest that SAC may act via antioxidant mechanisms to block NF-κB activation in Jurkat cells.