Involvement of sensory neuropeptides in the development of plasma extravasation in rat dorsal skin following thermal injury
1996; Wiley; Volume: 117; Issue: 6 Linguagem: Inglês
10.1111/j.1476-5381.1996.tb16698.x
ISSN1476-5381
Autores Tópico(s)Sexual function and dysfunction studies
ResumoBritish Journal of PharmacologyVolume 117, Issue 6 p. 1065-1070 Free Access Involvement of sensory neuropeptides in the development of plasma extravasation in rat dorsal skin following thermal injury L. Siney, Corresponding Author L. Siney Pharmacology Group, Biomedical Sciences Division, King's College, Manresa Road, London, SW3 6LXPharmacology Group, Biomedical Sciences Division, King's College, Manresa Road, London, SW3 6LXSearch for more papers by this authorS.D. Brain, S.D. Brain Pharmacology Group, Biomedical Sciences Division, King's College, Manresa Road, London, SW3 6LXSearch for more papers by this author L. Siney, Corresponding Author L. Siney Pharmacology Group, Biomedical Sciences Division, King's College, Manresa Road, London, SW3 6LXPharmacology Group, Biomedical Sciences Division, King's College, Manresa Road, London, SW3 6LXSearch for more papers by this authorS.D. Brain, S.D. Brain Pharmacology Group, Biomedical Sciences Division, King's College, Manresa Road, London, SW3 6LXSearch for more papers by this author First published: March 1996 https://doi.org/10.1111/j.1476-5381.1996.tb16698.xCitations: 35AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract 1 The involvement of the neuropeptides, substance P (SP) and calcitonin gene-related peptide (CGRP) in plasma extravasation following thermal injury of rat dorsal skin was investigated. 2 Heat applied to the dorsal skin of anaesthetized rats by a temperature-controlled skin heater (1 cm diameter) for 5 min induced temperature-dependent plasma protein extravasation at 46°C to 50°C measured over the 20 min following initiation of heat. 3 The NK1-receptor antagonist, SR140333, at doses above 36 nmol kg−1, significantly (P < 0.05) inhibited plasma extravasation by up to 79 ± 3% (120 nmol kg−1) after heat application at 48°C and by up to 53 ± 10% (120 nmol kg−1) after heat application at 50°C. 4 The CGRP1-receptor antagonist, CGRP8–37, at doses of 200 and 400 nmol kg−1, significantly inhibited (P < 0.01) plasma extravasation by 55 ± 9 and 60 ± 12%, respectively, after heat application at 48°C. At a dose of 200 nmol kg−1 CGRP8–37 inhibited plasma extravasation by 41 ± 8% after heat application at 50°C. 5 SR140333, 120 nmol kg−1, and CGRP8–37, 200 nmol kg−1 together significantly (P < 0.01) inhibited plasma extravasation by 84 ± 15% after heating at 48°C for 5 min. 6 In experiments where the response was measured for 0–5, 5–10, 10–15 or 15–20 min, SR140333, 120 nmol kg−1, significantly (P < 0.05) inhibited plasma extravasation which had accumulated during all the time periods measured. In comparison, CGRP8–37, 200 nmol kg−1, was significantly (P < 0.05) effective at time-points up to 15 min after initiation of injury. 7 In longer term experiments plasma protein extravasation continued for at least 95 min after initiation of thermal injury. SR140333, at a dose of 120 nmol kg−1, significantly inhibited plasma extravasation for up to 65 min after initiation of injury. 8 In conclusion, the data from the present study demonstrate that both SP and CGRP are likely to have a role in the acute plasma extravasation after thermal injury. In addition, evidence suggests SP may have a role in plasma extravasation for up to 65 min. References BLOMGREN, I. & BAGGE, U. (1984). Postburn blood flow, oedema, and survival of the hairy mouse ear after scald injury at different temperatures. Scand. J. Plast. Reconstr. Surg., 18, 269– 275. BRAIN, S.D. & WILLIAMS, T.J. (1985). Inflammatory oedema induced by synergism between calcitonin-gene related peptide (CGRP) and mediators of increased microvascular permeability. Br. J. Pharmacol., 86, 855– 860. DONOSO, V.S., FOURNIER, A., ST-PIERRE, S. & HUIDOBRO-TORO, P.J. (1990). 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