Peptide-imprinted polymer microspheres prepared by precipitation polymerization using a single bi-functional monomer
2009; Royal Society of Chemistry; Volume: 134; Issue: 4 Linguagem: Inglês
10.1039/b814967d
ISSN1364-5528
AutoresKeiichi Yoshimatsu, Jason LeJeune, David A. Spivak, Lei Ye,
Tópico(s)Analytical Chemistry and Chromatography
ResumoA single bi-functional monomer, N,O-bismethacryloyl ethanolamine (NOBE), was used in precipitation polymerization system to synthesize molecularly imprinted polymer (MIP) microspheres. Highly specific binding sites were obtained for N-terminal protected neuropeptides, Boc-Leu-enkephalin and Pyr-Leu-enkephalin. The use of NOBE allowed binding sites to be formed in polymer microspheres that are able to recognize target peptides through the consensus C-terminal sequence. The interesting molecular binding results suggest a new approach for peptide analysis combining in situ chemical modification with MIP recognition under non-aqueous conditions.
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