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Isotopic labeling-assisted metabolomics using LC–MS

2012; Springer Science+Business Media; Volume: 405; Issue: 1 Linguagem: Inglês

10.1007/s00216-012-6375-y

1618-2650

Christoph Bueschl, Rudolf Krska, Bernhard Kluger, Rainer Schuhmacher,

Mass Spectrometry Techniques and Applications

Metabolomics has emerged as the latest of the so-called "omics" disciplines and has great potential to provide deeper understanding of fundamental biochemical processes at the biological system level. Among recent technological developments, LC-HRMS enables determination of hundreds to thousands of metabolites over a wide range of concentrations and has developed into one of the most powerful techniques in non-targeted metabolomics. The analysis of mixtures of in-vivo-stable isotopic-labeled samples or reference substances with un-labeled samples leads to specific LC-MS data patterns which can be systematically exploited in practically all data-processing steps. This includes recognition of true metabolite-derived analytical features in highly complex LC-MS data and characterization of the global biochemical composition of biological samples. In addition, stable-isotopic labeling can be used for more accurate quantification (via internal standardization) and identification of compounds in different organisms.