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On the distribution of caffeic acid and the chlorogenic acid isomers in plants

1958; Elsevier BV; Volume: 74; Issue: 1 Linguagem: Inglês

10.1016/0003-9861(58)90207-8

1096-0384

Ernest Sondheimer,

Plant Physiology and Cultivation Studies

1. The silicic acid column technique described by Bulen et al. (9) has been used in a study of the distribution of caffeic acid and chlorogenic acid isomers in plants. Ultraviolet-absorption measurements and titration with alkali were used for the detection of these acids. 2. Resolution into five bands was achieved. Four of these bands had peak effluent volumes that coincided with caffeic acid, isochlorogenic acid, chlorogenic acid, and neochlorogenic acid. Pseudochlorogenic acid was not detected. Free caffeic acid appears to be absent or only a minor component in most plants. Of the eleven plant sources examined, six had the chlorogenic acid band as the major caffeic acid derivative. Only in sweet-potato peelings did the isochlorogenic acid band account for more than 50 % of the total. In all the members of the Prunus family studied, the neochlorogenic acid band comprised the greatest fraction of the chlorogenic acid isomers. 3. One band which was eluted between chlorogenic acid and neochlorogenic acid may be a new caffeic acid derivative and has been designated "Band 510." This material was isolated from green coffee beans as a noncrystalline solid. 4. The silicic acid column has been used for the isolation of crystalline chlorogenic acid from blueberry leaves.