Saline-soluble preparations of deoxyribonucleoproteins
1964; Elsevier BV; Volume: 106; Linguagem: Inglês
10.1016/0003-9861(64)90222-x
ISSN1096-0384
AutoresWilliam A. Atchley, N.V. Bhagavan,
Tópico(s)Metal complexes synthesis and properties
ResumoThe solubility of crude deoxyribonucleoprotein (DNP) extracted from malignant mammalian cells was measured in varying concentrations of the salts of monovalent and divalent cations. NaCl, most effective at about 0.12 M, precipitated less DNP than did MgCl2, which was most effective at about 0.01 M. DNA from the NaCl-insoluble DNP was fibrous and constituted about 80% of the total DNA; DNA from the NaCl-soluble, MgCl2-insoluble DNP was nonfibrous and constituted about 20% of the total DNA. The precipitation of both types of DNP was inhibited by the aminocarboxylic acids glycine, β-alanine, γ-aminobutyric acid, and ϵ-aminocaproic acid, in order of increasing effectiveness. ω-Aminocaprylic acid was somewhat less effective than ϵ-aminocaproic acid. The alpha isomers of the aminocarboxylic acids studied were for the most part ineffective. The aminocarboxylic acids did not act as salts with respect to DNP, nor was their solubilizing action related to their ability to chelate divalent cations. The effect appeared to correlate with the dielectric increments of the aminocarboxylic acids.
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