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ESR studies on membrane fluidity of Chinese hamster ovary cells grown on microcarriers and in suspension

1980; Elsevier BV; Volume: 130; Issue: 2 Linguagem: Inglês

10.1016/0014-4827(80)90022-1

1090-2422

Chengcai Lai, Larry E. Hopwood, Harold M. Swartz,

Microfluidic and Bio-sensing Technologies

Electron spin resonance (ESR) spin label methods were used to study membrane fluidity of Chinese hamster ovary (CHO) cells grown on microcarriers and in suspension using 5-doxylstearic acid spin label as a probe. CHO cells grown on microcarriers had a more rigid cell membrane compared to CHO cells grown in suspension culture. CHO cells removed from the surface of the microcarriers by either trypsinization, EDTA treatment or osmotic shock had a membrane fluidity similar to that of CHO cells grown in suspension culture. Conversely, when the cells grown in suspension culture were attached and flattened on the surface of the microcarriers the fluidity decreased. Moreover, membrane fluidity of CHO cells grown on microcarriers changed as a function of the population density, whereas that of the cells in suspension did not. This implies that cell adhesion and/or cell-cell interactions influence the fluidity of the cell surface membrane.