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Establishment of Alb-DsRed2 transgenic rat for liver regeneration research

2003; Elsevier BV; Volume: 311; Issue: 2 Linguagem: Inglês

10.1016/j.bbrc.2003.09.230

1090-2104

Yuki Sato, Yuka Igarashi, Yoji Hakamata, Takashi Murakami, Takashi Kaneko, Masafumi Takahashi, Norimasa Seo, Eiji Kobayashi,

Cancer Research and Treatments

Because serum albumin is specifically produced by mature hepatocytes, detection system of albumin producing cells could be a valuable tool to visualize liver regeneration or development. We have developed here an albumin enhancer/promoter-driven Alb-DsRed2 Tg rat that expresses DsRed2, having liver-specific reporter gene expression of red fluorescent protein. To study the transdifferentiation of bone marrow cells (BMCs) into albumin producing cells, BMCs from the Alb-DsRed2 Tg rat were injected into rats having acute liver damage caused by 2-acetylaminofluorene plus carbon tetrachloride and chronic liver damage by repeated administration of CCl4. DsRed2-positive cells were generated in the recipient liver after BMC injection. The number of transdifferentiated DsRed2-positive cells in chronic liver injury model was increased comparing with that in acute injury model. We propose that the Alb-DsRed2 Tg rat is well suited to studying in vivo liver regeneration.