Voltar
Artigo Produção Nacional Revisado por pares
BIBTEX RIS

Effects of L-Nitro-Arginine Methyl Ester, an Inhibitor of Nitric Oxide Biosynthesis, on Intestinal Ischemia/Reperfusion Injury in Rabbits

2010; Elsevier BV; Volume: 42; Issue: 2 Linguagem: Inglês

10.1016/j.transproceed.2010.01.037

ISSN

1873-2623

Autores

Murched Omar Taha, Regiane Miranda‐Ferreira, Anna Luiza Negrini Fagundes, Djalma José Fagundes, Ricardo Santos Simões, Joilson Moura dos Santos, PF de Souza, Itamar Souza de Oliveira-Júnior, Antonio Marchini, Isabella Gomes, Hugo P. Monteiro, L.O. Mendonça, Afonso Caricati‐Neto,

Tópico(s)

Anesthesia and Neurotoxicity Research

Resumo

To study whether treatment with L-nitro-arginine methyl ester (L-NAME), an inhibitor of nitric oxide biosynthesis, attenuates intestinal dysfunction caused by ischemia (I) and/or reperfusion (R), rabbits were treated with L-NAME (15 mgxkg(-1), intervenously) or saline olution (SS) prior to I (60 minutes) induced by occlusion of superior mesenteric artery and/or R (120 minutes). After I or I/R, isolated jejunal segments (2 cm) were mounted in an organ bath to study nerve-mediated contractions stimulated by electrical pulses or KCI using a digital recording system. Thin jejunal slices were stained (hematoxylin and eosin) for analysis by optical microscopy. Compared with a sham group, the jejunal contractions were similar in the I/R + L-NAME, but reduced in I + SS, I/R + SS, and I + L-NAME groups. The jejunal enteric nerves were damaged in the I + SS, I/R + SS, and I + L-NAME cohorts, but not among the I/R + L-NAME cohort. These results suggested that L-NAME attenuated intestinal dysfunction caused by R but not by I.

Referência(s)