AUTOLOGOUS TRANSPLANTATION OF UROTHELIUM INTO DEMUCOSALIZED GASTROINTESTINAL SEGMENTS: EVIDENCE FOR EPITHELIALIZATION AND DIFFERENTIATION OF IN VITRO EXPANDED AND TRANSPLANTED UROTHELIAL CELLS
1998; Lippincott Williams & Wilkins; Volume: 159; Issue: 1 Linguagem: Inglês
10.1016/s0022-5347(01)64083-7
ISSN1527-3792
AutoresBirgit M. Schaefer, Christian Lorenz, Walter Back, R. Moll, Tung‐Tien Sun, C. Schöber, K.-L. Waag, Michael D. Kramer,
Tópico(s)Urological Disorders and Treatments
ResumoNo AccessJournal of UrologyInvestigative Urology1 Jan 1998AUTOLOGOUS TRANSPLANTATION OF UROTHELIUM INTO DEMUCOSALIZED GASTROINTESTINAL SEGMENTS: EVIDENCE FOR EPITHELIALIZATION AND DIFFERENTIATION OF IN VITRO EXPANDED AND TRANSPLANTED UROTHELIAL CELLS Birgit M. Schaefer, Christian Lorenz, Walter Back, Roland Moll, Tung-Tien Sun, Claudia Schober, Karl-Ludwig Waag, and Michael D. Kramer Birgit M. SchaeferBirgit M. Schaefer More articles by this author , Christian LorenzChristian Lorenz More articles by this author , Walter BackWalter Back More articles by this author , Roland MollRoland Moll More articles by this author , Tung-Tien SunTung-Tien Sun More articles by this author , Claudia SchoberClaudia Schober More articles by this author , Karl-Ludwig WaagKarl-Ludwig Waag More articles by this author , and Michael D. KramerMichael D. Kramer More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)64083-7AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Our study established a technique for in vitro expansion and subsequent transplantation of autologous urothelial cells into vascularized seromuscular segments from stomach and colon in sheep. The proof of proliferation and differentiation of the transplanted urothelium in the absence of resident urothelium is considered to be a prerequisite for use of this technique in bladder augmentation. Materials and Methods: Autologous sheep urothelial cells were expanded in vitro and grown on collagen membranes for sheet grafting. Using a vital stain, viability and confluency status of the urothelial graft were determined before transplantation into demucosalized segments isolated from the sheep stomach and colon gastrointestinal pouches. The gastrointestinal segments were sewn up and remained in the abdomen as small pouches stiched to the abdominal wall. Take and differentiation of transplanted cells within the pouch were assessed two and three weeks later using histological and immunohistological means. Results: Urothelial cells grew well on collagen membranes. A confluency status > 40% and co-culturing with 3T3 feeder cells favored successful transplantation. Two weeks after transplantation a multilayered urothelial-like epithelium was found to line the lumen of the pouch. The epithelium was characterized by a distinct urothelium-typical distribution of basal and luminal keratins and the expression of the umbrella cell-specific marker uroplakin III. Moreover, the epithelium had an underlying basal lamina which focally contained collagen type IV. Conclusions: The data indicate that in vitro expanded urothelial cells are capable of epithelializing demucosalized gastrointestinal segments forming a genuine, differentiated “neo” urothelium. References 1 : Optimization of uretero-intestinal anastomosis in urinary diversion: an experimental study in dogs II. Influence of exposure to urine on the healing of the ureter and ileum.. Urol. Res.1993; 21: 131. Google Scholar 2 : The syndrome of dysuria and hematuria in pediatric urinary reconstruction with stomach.. J. Urol.1993; 150: 707. Abstract, Google Scholar 3 : Demucosalized augmentation gastrocystoplasty with bladder autoaugmentation in pediatric patients.. J. Urol.1996; 156: 206. Abstract, Google Scholar 4 : Metabolic consequences of sigmoidocystoplasty in children.. Pediatr. Surg. Int.1996; 11: 150. Google Scholar 5 : Complications of bowel augmentation.. Dial. Pediatr. Urol.1995; 18: 2. Google Scholar 6 : Malignancies in bladder augmentations and intestinal conduits.. J. Urol.1990; 143: 671. Abstract, Google Scholar 7 : Long-term histopathological changes observed in rats subjected to augmentation cystoplasty.. J. Urol.1994; 152: 720. Abstract, Google Scholar 8 : Acid-base changes following urinary tract reconstruction for continent diversion and orthotopic bladder replacement.. J. Urol.1994; 152: 338. Abstract, Google Scholar 9 : Lithogenic properties of enterocystoplasty.. J. Urol.1992; 148: 575. Abstract, Google Scholar 10 : Histological and bacteriological findings in long-term ileocystoplasty and coloncystoplasty in the rat.. J. Urol.1993; 150: 1321. Abstract, Google Scholar 11 : Reconstruction of the bladder with a seromuscular graft.. J. Urol.1957; 78: 236. Abstract, Google Scholar 12 : The use of inverted seromuscular grafts from the ileum and colon for reconstruction of the urinary bladder.. Ann. Surg.1954; 140: 832. Google Scholar 13 : Uroepithelial lined ileal segments as a bladder replacement: experimental observations and brief review of literature.. J. Urol.1959; 82: 633. Abstract, Google Scholar 14 : The feasibility of preparing an ideal substitute for the urinary bladder.. Ann. Royal. Coll. Surg.1964; 35: 287. Google Scholar 15 : Heterotopic ossification in uroepithelial regeneration on grafts of ileum and colon.. Surg. For. Urol.1961; 12: 498. Google Scholar 16 : Augmentation cytoplasty utilizing deepithelialized sigmoid colon: a preliminary study.. J. Urol.1990; 143: 1257. Abstract, Google Scholar 17 : Urothelial lined colocystoplasty in a sheep model.. Eur. Urol.1994; 26: 240. Google Scholar 18 : Urothelium in seromuscular colocystoplasty.. Urology1995; 46: 443. Google Scholar 19 : Enterocystoplasty using modified pedicled, detubularized, de-epithelialized sigmoid patches in the mini-pig model.. J. Urol.1995; 154: 893. Abstract, Google Scholar 20 : Augmentation cystoplasty using pedicled and de-epithelialized gastric patches in the mini-pig model.. J. Urol.1996; 156: 608. Abstract, Google Scholar 21 : Cultured urothelium in sheep bladder augmentation.. Pediatr. Surg. Int.1996; 11: 456. Google Scholar 22 : Grafting of cultured uroepithelium and bladder mucosa into de-epithelialized segments of colon in rabbits.. J. Urol.1994; 152: 671. Abstract, Google Scholar 23 : Seromuscular colocystoplasty lined with urothelium: experimental study.. Urology1994; 44: 743. Google Scholar 24 : Autoaugmentation gastrocystoplasty and demucosalized gastrocystoplasty in a sheep model.. Urology1995; 45: 291. Google Scholar 25 : Effect of seromuscular colocystoplasty lined with urothelium and partial detrusorectomy on a new canine model of reduced bladder capacity.. J. Urol.1996; 156: 903. Google Scholar 26 : Urothelial tissue culture for bladder reconstruction: an experimental study.. J. Urol.1993; 150: 721. Abstract, Google Scholar 27 : Phenotypic and cytogenetic characterization of human bladder urothelia expanded in vitro.. J. Urol.1994; 152: 665. Abstract, Google Scholar 28 : Methods in laboratory investigation. Normal human urothelial cells in vitro: proliferation and induction of stratification.. Lab. Invest.1994; 71: 583. Google Scholar 29 : Culture and characterisation of human urothelium in vivo and in vitro.. Urol. Res.1994; 22: 67. Google Scholar 30 : Tissue engineering in the urinary tract.. Dial. Pediat. Urol.1995; 18: 6. Google Scholar 31 : Serial cultivation of strains of human keratinocytes: the formation of keratinizing colonies from single cells.. Cell1975; 6: 331. Google Scholar 32 : Uroplakins, specific membrane proteins of urothelial umbrella cells, as histological markers of metastatic transitional cell carcinomas.. Am. J. Pathol.1995; 147: 1383. Google Scholar 33 : Mammalian uroplakins: a group of highly conserved urothelial differentiation-related membrane proteins.. J. Biol. Chem.1994; 269: 13716. Google Scholar 34 : Influence of extracellular matrix proteins on human keratinocyte attachment, proliferation and transfer to a dermal wound model.. Burns1996; 22: 93. Google Scholar 35 : Modulation of IL-6 production and IL-1 activity by keratinocyte-fibroblast interaction.. J. Invest. Dermatol.1993; 101: 316. Google Scholar 36 : Integrin expression and differentiation in transformed human epidermal cells is regulated by fibroblasts.. J. Cell Sci.1992; 103: 755. Google Scholar 37 : Keratinocytes stimulate prostaglandin I2 synthesis by 3T3 cells and exhibits enhanced cornification when exposed to protaglandin I2 analogues.. J. Cell. Physiol.1992; 150: 269. Google Scholar 38 : Implantation in vivo and retrieval of artificial structures consisting of rabbit and human urothelium and human bladder muscle.. J. Urol.1993; 150: 608. Abstract, Google Scholar 39 : Cytokeratins in normal and malignant transitional epithelium: Maintenance of expression of urothelial differentiation features in transitional cell carcinomas and bladder carcinoma cell culture lines.. Am. J. Pathol.1988; 132: 123. Google Scholar 40 : Use of biodegradable mesh as a transport for cultured uroepithelial graft: an improved method using collagen gel.. Urology1994; 44: 139. Google Scholar 41 : An in vitro model of urothelial regeneration: effects of growth factors and extracellular matrix proteins.. J. Pathol.1994; 173: 283. Google Scholar 42 : Quantitative evaluation of the growth of established cell lines on the surface of collagen, collagen composite and reconstituted basement membrane.. Urol., Res.1992; 20: 285. Google Scholar 43 : Reconstruction of the urinary bladder mucosa in three-dimensional collagen gel culture: fibroblast-extracellular matrix interactions on the differentiation of transitional epithelial cells.. J. Urol.1995; 153: 2060. Abstract, Google Scholar 44 : Formation of urothelial structures in vivo from dissociated cells attached to biodegradable polymer scaffolds in vitro.. J. Urol.1992; 148: 658. Link, Google Scholar 45 : Growth kinetics and differentiation in vitro of normal human uroepithelial cells on collagen gel substrates in defined medium.. J. Cell. Physiol.1987; 131: 285. Google Scholar 46 : Ultrastructural and immunohistochemical characterization of basal cells in three-dimensional culture models of the skin.. Arch. Dermatol. Res.1994; 286: 53. Google Scholar 47 : Evaluation of a porous bovine collagen membrane bandage for management of wounds in horses.. Am. J. Vet. Res.1995; 56: 1663. Google Scholar 48 : Preliminary evaluation of a technique for inhibiting intimal hyperplasia: implantation of a resorbable luminal collagen membrane.. Ann. Vasc. Surg.1995; 9: 135. Google Scholar 49 : Allogeneic fibroblasts used to grow cultured epidermal autografts persist in vivo and sensitize the graft recipient for accelerated second-set rejection.. J. Trauma.1996; 41: 51. Google Scholar 50 : Rejection of cultured keratinocyte allografts in presensitized mice.. Transplantation1993; 56: 265. Google Scholar 51 : Xenogeneic mouse fibroblasts persist in human cultured epidermal grafts: a possible mechanism of graft loss.. J. Trauma.1995; 39: 75. Google Scholar 52 : Intestinal de-epithelialization and augmentation cystoplasty: an animal model.. Urology1995; 46: 36. Google Scholar From the University Institute for Immunology, Laboratory for Immunopathology, Heidelberg, Department of Pediatric Surgery of the University Hospital Mannheim, Department of Pathology of the University Hospital Mannheim, Institute of Pathology of the Martin-Luther-University Halle-Wittenberg, Halle, Germany, and the Epithelial Biology Unit, Department of Dermatology, New York University Medical Center, New York, New YorkAccepted for publication August 18, 1997(Schaeffer) Requests for reprints: Institut fur Immunologie und Serologie, der Universit at Heidelberg, INF 305, D-69120 Heidelberg, GermanySupported by Deutsche Forschungsgemeinschaft No. Lo 510/2-1 and the Faculty of Medicine, University Hospital Mannheim, No. FA 55/95© 1998 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byHAFEZ A, AFSHAR K, BÄGLI D, BAHORIC A, AITKEN K, SMITH C and KHOURY A (2018) AEROSOL TRANSFER OF BLADDER UROTHELIAL AND SMOOTH MUSCLE CELLS ONTO DEMUCOSALIZED COLONIC SEGMENTS FOR PORCINE BLADDER AUGMENTATION IN VIVO: A 6-WEEK EXPERIMENTAL STUDYJournal of Urology, VOL. 174, NO. 4 Part 2, (1663-1668), Online publication date: 1-Oct-2005.HAFEZ A, BÄGLI D, BAHORIC A, AITKEN K, SMITH C, HERZ D and KHOURY A (2018) Aerosol Transfer of Bladder Urothelial and Smooth Muscle Cells Onto Demucosalized Colonic Segments: A Pilot StudyJournal of Urology, VOL. 169, NO. 6, (2316-2320), Online publication date: 1-Jun-2003.SCHOELLER T, LILLE S, STENZL A, NINKOVIĆ M, PIZA H, OTTO A, RUSSELL R and WECHSELBERGER G (2018) BLADDER RECONSTRUCTION USING A PREVASCULARIZED CAPSULAR TISSUE SEEDED WITH UROTHELIAL CELLSJournal of Urology, VOL. 165, NO. 3, (980-985), Online publication date: 1-Mar-2001. Volume 159Issue 1January 1998Page: 284-290 Advertisement Copyright & Permissions© 1998 by American Urological Association, Inc.MetricsAuthor Information Birgit M. Schaefer More articles by this author Christian Lorenz More articles by this author Walter Back More articles by this author Roland Moll More articles by this author Tung-Tien Sun More articles by this author Claudia Schober More articles by this author Karl-Ludwig Waag More articles by this author Michael D. Kramer More articles by this author Expand All Advertisement PDF downloadLoading ...
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