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AUTOLOGOUS TRANSPLANTATION OF UROTHELIUM INTO DEMUCOSALIZED GASTROINTESTINAL SEGMENTS: EVIDENCE FOR EPITHELIALIZATION AND DIFFERENTIATION OF IN VITRO EXPANDED AND TRANSPLANTED UROTHELIAL CELLS

1998; Lippincott Williams & Wilkins; Volume: 159; Issue: 1 Linguagem: Inglês

10.1016/s0022-5347(01)64083-7

1527-3792

Birgit M. Schaefer, Christian Lorenz, Walter Back, R. Moll, Tung‐Tien Sun, C. Schöber, K.-L. Waag, Michael D. Kramer,

Urological Disorders and Treatments

No AccessJournal of UrologyInvestigative Urology1 Jan 1998AUTOLOGOUS TRANSPLANTATION OF UROTHELIUM INTO DEMUCOSALIZED GASTROINTESTINAL SEGMENTS: EVIDENCE FOR EPITHELIALIZATION AND DIFFERENTIATION OF IN VITRO EXPANDED AND TRANSPLANTED UROTHELIAL CELLS Birgit M. Schaefer, Christian Lorenz, Walter Back, Roland Moll, Tung-Tien Sun, Claudia Schober, Karl-Ludwig Waag, and Michael D. Kramer Birgit M. SchaeferBirgit M. Schaefer More articles by this author , Christian LorenzChristian Lorenz More articles by this author , Walter BackWalter Back More articles by this author , Roland MollRoland Moll More articles by this author , Tung-Tien SunTung-Tien Sun More articles by this author , Claudia SchoberClaudia Schober More articles by this author , Karl-Ludwig WaagKarl-Ludwig Waag More articles by this author , and Michael D. KramerMichael D. Kramer More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)64083-7AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Our study established a technique for in vitro expansion and subsequent transplantation of autologous urothelial cells into vascularized seromuscular segments from stomach and colon in sheep. The proof of proliferation and differentiation of the transplanted urothelium in the absence of resident urothelium is considered to be a prerequisite for use of this technique in bladder augmentation. Materials and Methods: Autologous sheep urothelial cells were expanded in vitro and grown on collagen membranes for sheet grafting. Using a vital stain, viability and confluency status of the urothelial graft were determined before transplantation into demucosalized segments isolated from the sheep stomach and colon gastrointestinal pouches. The gastrointestinal segments were sewn up and remained in the abdomen as small pouches stiched to the abdominal wall. Take and differentiation of transplanted cells within the pouch were assessed two and three weeks later using histological and immunohistological means. Results: Urothelial cells grew well on collagen membranes. A confluency status > 40% and co-culturing with 3T3 feeder cells favored successful transplantation. Two weeks after transplantation a multilayered urothelial-like epithelium was found to line the lumen of the pouch. The epithelium was characterized by a distinct urothelium-typical distribution of basal and luminal keratins and the expression of the umbrella cell-specific marker uroplakin III. Moreover, the epithelium had an underlying basal lamina which focally contained collagen type IV. 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Google Scholar From the University Institute for Immunology, Laboratory for Immunopathology, Heidelberg, Department of Pediatric Surgery of the University Hospital Mannheim, Department of Pathology of the University Hospital Mannheim, Institute of Pathology of the Martin-Luther-University Halle-Wittenberg, Halle, Germany, and the Epithelial Biology Unit, Department of Dermatology, New York University Medical Center, New York, New YorkAccepted for publication August 18, 1997(Schaeffer) Requests for reprints: Institut fur Immunologie und Serologie, der Universit at Heidelberg, INF 305, D-69120 Heidelberg, GermanySupported by Deutsche Forschungsgemeinschaft No. Lo 510/2-1 and the Faculty of Medicine, University Hospital Mannheim, No. FA 55/95© 1998 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byHAFEZ A, AFSHAR K, BÄGLI D, BAHORIC A, AITKEN K, SMITH C and KHOURY A (2018) AEROSOL TRANSFER OF BLADDER UROTHELIAL AND SMOOTH MUSCLE CELLS ONTO DEMUCOSALIZED COLONIC SEGMENTS FOR PORCINE BLADDER AUGMENTATION IN VIVO: A 6-WEEK EXPERIMENTAL STUDYJournal of Urology, VOL. 174, NO. 4 Part 2, (1663-1668), Online publication date: 1-Oct-2005.HAFEZ A, BÄGLI D, BAHORIC A, AITKEN K, SMITH C, HERZ D and KHOURY A (2018) Aerosol Transfer of Bladder Urothelial and Smooth Muscle Cells Onto Demucosalized Colonic Segments: A Pilot StudyJournal of Urology, VOL. 169, NO. 6, (2316-2320), Online publication date: 1-Jun-2003.SCHOELLER T, LILLE S, STENZL A, NINKOVIĆ M, PIZA H, OTTO A, RUSSELL R and WECHSELBERGER G (2018) BLADDER RECONSTRUCTION USING A PREVASCULARIZED CAPSULAR TISSUE SEEDED WITH UROTHELIAL CELLSJournal of Urology, VOL. 165, NO. 3, (980-985), Online publication date: 1-Mar-2001. Volume 159Issue 1January 1998Page: 284-290 Advertisement Copyright & Permissions© 1998 by American Urological Association, Inc.MetricsAuthor Information Birgit M. Schaefer More articles by this author Christian Lorenz More articles by this author Walter Back More articles by this author Roland Moll More articles by this author Tung-Tien Sun More articles by this author Claudia Schober More articles by this author Karl-Ludwig Waag More articles by this author Michael D. Kramer More articles by this author Expand All Advertisement PDF downloadLoading ...