DNA Integrity and DNA Polymerase Activity in Deteriorated Maize Embryo Axes
1988; Elsevier BV; Volume: 133; Issue: 5 Linguagem: Inglês
10.1016/s0176-1617(88)80014-2
ISSN1618-1328
AutoresJorge Manuel Vázquez Ramos, Sergio López, Edgar Vázquez, Ezequiel Murillo,
Tópico(s)Plant tissue culture and regeneration
ResumoThe storage of maize seeds for short periods of time under improper, dry (60 °C, 2 d), and humid (40 °C, 6 d, 100 % relative humidity) conditions causes a significant decrease in viability. This decrease occurs concomitantly with breakage of DNA. When embryo axes from these seeds are put to germinate, reduction in DNA synthesis capacity can be observed. The treatment of seeds affects polymerase activity differently since recovery is faster after humid treatment than after dry treatment and appears to be due to new enzyme synthesis since recovery is inhibited by cycloheximide. It is suggested that DNA polymerase plays an important role during the first hours of seed germination.
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