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THE SIGNIFICANCE OF THE DIFFERENCE IN BACTERIAL ADHERENCE BETWEEN BLADDER AND ILEUM USING RAT ILEAL AUGMENTED BLADDER

1999; Lippincott Williams & Wilkins; Volume: 162; Issue: 1 Linguagem: Inglês

10.1097/00005392-199907000-00075

1527-3792

Yuzo Nakano, Masato Fujisawa, Takashi Matsui, Soichi Arakawa, Sadao Kamidono,

Urinary Tract Infections Management

No AccessJournal of UrologyInvestigative Urology1 Jul 1999THE SIGNIFICANCE OF THE DIFFERENCE IN BACTERIAL ADHERENCE BETWEEN BLADDER AND ILEUM USING RAT ILEAL AUGMENTED BLADDER YUZO NAKANO, MASATO FUJISAWA, TAKASHI MATSUI, SOICHI ARAKAWA, and SADAO KAMIDONO YUZO NAKANOYUZO NAKANO More articles by this author , MASATO FUJISAWAMASATO FUJISAWA More articles by this author , TAKASHI MATSUITAKASHI MATSUI More articles by this author , SOICHI ARAKAWASOICHI ARAKAWA More articles by this author , and SADAO KAMIDONOSADAO KAMIDONO More articles by this author View All Author Informationhttps://doi.org/10.1097/00005392-199907000-00075AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Objectives: Intestinal segments are frequently used in the reconstruction of the urinary tract. Chronic bacteriuria is frequently observed in these patients, but the reason is not clearly understood. Therefore, we studied the difference in bacterial adherence between bladder and ileum using the rat ileal augmented bladder model to investigate the cause of chronic bacteriuria. Materials and Methods: Augmentation of the bladder using ileum and a sham operation were performed under sodium pentobarbital in 102 and 10 Sprague-Dawley rats, respectively. At three months after the operation, urinary pH and plasma concentration of sodium, chloride and potassium were measured and urinary culture was done. Urovirulence factors of Escherichia coli aspirated from augmented bladder were detected by polymerase chain reaction (PCR). Five to six rats with negative urinary cultures after the augmentation were used for each experimental cystitis. E. coli with type I pili aspirated from augmented rats and three clinically isolated strains of E. coli, C5 (type I pili, aerobactin), C92 (type I pili, aerobactin, P fimbriae), and C189 (type I pili, aerobactin, P fimbriae, CNF), were transurethrally inoculated into the augmented bladder of rats. Fourteen days after inoculation, rats were sacrificed and colony-forming units (CFU) per mg. of tissue of bladder and ileum were measured. Results: After operation, urinary pH and the serum level of chloride in all augmented groups were higher than those of the controls. Bacterial colonization was observed in 56 of 89 rats. Most of them ware E. coli having only type I pili as a virulence factor. In contrast, the sham operated group revealed no bacterial colonization. In experimental cystitis, E. coli with only type I pili aspirated from augmented rats and E. coli C5 were clearly adhered to ileum rather than to bladder, but E. coli C92 and C189 showed no significant difference with respect to adherence to the two tissues. In experimental cystitis II, E. coli C5 with D-mannose were washed out in 3 of 5 rats by 14 days, while E. coli C5 without D-mannose were not washed out in all rats by 14 days. Conclusions: These results suggested that the difference in bacterial adherence due to urovirulence factors, especially type I pili, is one of the main causes of asymptomatic bacteriuria after urinary reconstruction. References 1 : Detection of urovirulence factors in Escherichia coli by multiplex polymerase chain reaction. FEMS Immunol. Med. Microbiol.1995; 12: 85. Google Scholar 2 : Assessment of significance of virulence factors of uropathogene Escherichia coli in experimental urinary tract. Microbiol. Immunol.1996; 40: 607. Google Scholar 4 : Morphologic and functional alterations of intestinal segments following urinary diversion. J. Urol.1993; 149: 664. Link, Google Scholar 5 : Adherence of uropathogenic E. coli to differentiated human uroepithelial cells grown in vitro. J. Urol.1990; 143: 146. Link, Google Scholar 6 : Mechanism of solute transport following urinary diversion through intestinal segments: an experiment study with rats. J. Urol.1991; 146: 1390. Abstract, Google Scholar 7 : Inhibition of transport processes of intestinal segments following augmentation enterocystoplasty in rats. J. Urol.1996; 156: 1872. Link, Google Scholar 8 : Urinary pH and urea concentration correlate to the bacterial colonization rate in gastric, colonic, ileal and myoperitoneal bladder augmentation. J. Urol.1995; 154: 899. Link, Google Scholar 9 : Virulence factors in Escherichia coli urinary tract infection. Clin. Microbiol. Rev.1991; 4: 80. Google Scholar 10 : Identification of the specific oligosaccharide sites recognized by type I fimbriae from Escherichia coli on nonspecific cross-reacting antigen, a CD66 cluster granulocyte glycoprotein. J. Biol. Chem.1993; 268: 15510. Google Scholar 11 : Long-term histopathologic changes observed in rats subjected to augmentation cystoplasty. J. Urol.1994; 152: 720. Google Scholar 14 : The papG-adhesin at the tip of P-fimbriae provides Escherichia coli with a competitive edge in experimental bladder infection of cynomolgus monkeys. J. Exp. Med.1995; 182: 1695. Google Scholar 15 : Blood and urine analysis in patients with intestinal bladders. Br. J. Urol.1991; 68: 311. Google Scholar 16 : Techniques and complications of substitution and augmentation cystplasty. Scand. J. Urol. Nephrol.1992; 142: 123. Google Scholar From the Department of Urology, Kobe University School of Medicine, Kobe, Japan(Nakano) Requests for reprints: Department of Urology, Kobe University School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017 Japan.© 1999 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byMiura T, Tanaka K, Nakano Y, Arakawa S, Takenaka A and Fujisawa M (2018) The Impact of Decreasing Urinary IgA Levels on Decreased Bacteriuria in a Rat Model of Ileal Augmented BladderJournal of Urology, VOL. 181, NO. 1, (372-378), Online publication date: 1-Jan-2009.KEEGAN S, GRAHAM C, NEAL D, BLUM-OEHLER G, N’DOW J, PEARSON J and GALLY D (2018) Characterization of Escherichia Coli Strains Causing Urinary Tract Infections in Patients With Transposed Intestinal SegmentsJournal of Urology, VOL. 169, NO. 6, (2382-2387), Online publication date: 1-Jun-2003.SAKAI Y, FUJISAWA M, NAKANO Y, MIYAZAKI S, ARAKAWA S and KAMIDONO S (2018) BACTERIAL ADHERENCE IN A RAT BLADDER AUGMENTATION MODEL: : ILEOCYSTOPLASTY VERSUS COLOCYSTOPLASTYJournal of Urology, VOL. 164, NO. 6, (2104-2107), Online publication date: 1-Dec-2000. Volume 162Issue 1July 1999Page: 243-247 Advertisement Copyright & Permissions© 1999 by American Urological Association, Inc.MetricsAuthor Information YUZO NAKANO More articles by this author MASATO FUJISAWA More articles by this author TAKASHI MATSUI More articles by this author SOICHI ARAKAWA More articles by this author SADAO KAMIDONO More articles by this author Expand All Advertisement PDF downloadLoading ...