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Penicillin productivity and glutathione-dependent detoxification of phenylacetic and phenoxyacetic acids inPenicillium chrysogenum

2001; Wiley; Volume: 41; Issue: 2 Linguagem: Inglês

10.1002/1521-4028(200105)41

1521-4028

Tamás Emri, Éva Leiter, Etelka Farkas, István Pócsi,

Metal-Catalyzed Oxygenation Mechanisms

Journal of Basic MicrobiologyVolume 41, Issue 2 p. 67-73 Original Paper Penicillin productivity and glutathione-dependent detoxification of phenylacetic and phenoxyacetic acids in Penicillium chrysogenum Tamás Emri, Tamás Emri Department of Microbiology and Biotechnology, Faculty of Science, University of Debrecen, P.O. Box 21., H-4010 Debrecen, HungarySearch for more papers by this authorÉva Leiter, Éva Leiter Department of Microbiology and Biotechnology, Faculty of Science, University of Debrecen, P.O. Box 21., H-4010 Debrecen, HungarySearch for more papers by this authorEtelka Farkas, Etelka Farkas Department of Inorganic and Analytical Chemistry, Faculty of Science, University of Debrecen, P.O. Box 21., H-4010 Debrecen, HungarySearch for more papers by this authorIstván Pócsi Dr., István Pócsi Dr. [email protected] Department of Microbiology and Biotechnology, Faculty of Science, University of Debrecen, P.O. Box 21., H-4010 Debrecen, HungarySearch for more papers by this author Tamás Emri, Tamás Emri Department of Microbiology and Biotechnology, Faculty of Science, University of Debrecen, P.O. Box 21., H-4010 Debrecen, HungarySearch for more papers by this authorÉva Leiter, Éva Leiter Department of Microbiology and Biotechnology, Faculty of Science, University of Debrecen, P.O. Box 21., H-4010 Debrecen, HungarySearch for more papers by this authorEtelka Farkas, Etelka Farkas Department of Inorganic and Analytical Chemistry, Faculty of Science, University of Debrecen, P.O. Box 21., H-4010 Debrecen, HungarySearch for more papers by this authorIstván Pócsi Dr., István Pócsi Dr. [email protected] Department of Microbiology and Biotechnology, Faculty of Science, University of Debrecen, P.O. Box 21., H-4010 Debrecen, HungarySearch for more papers by this author First published: 05 June 2001 https://doi.org/10.1002/1521-4028(200105)41:2 3.0.CO;2-JCitations: 8AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Both toxicity and penicillin productivity of the hydroxylated derivatives of phenylacetic acid (PA) and phenoxyacetic acid (POA) were highly dependent on the position of hydroxylation on the aromatic ring in Penicillium chrysogenum. Hydroxylation at position 2 diminished penicillin production but the compounds retained most of their toxicity. On the other hand, hydroxylation at position 4 resulted in barely toxic derivatives with still significant penicillin productivity. 3-Hydroxy-PA was a weak side-chain precursor with considerably reduced toxicity. The activity of the glutathione-dependent detoxification pathway correlated well with the toxicity of the compounds but there was no correlation between acidity, toxicity and penicillin productivity. References Alonso, M.J., Bermejo, F., Reglero, A., Fernández-Cañón, J.M., González de Buitrago, G. and Luengo, J.M., 1988. Enzymatic synthesis of penicillins. J. Antibiotics, 41, 1074–1084. Anderson, M.E., 1985, Determination of glutathione and glutathione disulphide in biological samples. Methods Enzymol., 113, 548–555. Bundgaard, H. , andIlver, K.A., 1972. A new spectrophotometric method for the determination of penicillins. J. Pharm. Pharmac., 24, 790–794. 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