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Antibacterial action of chitosan and carboxymethylated chitosan

2000; Wiley; Volume: 79; Issue: 7 Linguagem: Inglês

10.1002/1097-4628(20010214)79

1097-4628

Xiaofei Liu, Yun Guan, Dong Zhi Yang, Zhi Li, Yao Kang,

Polydiacetylene-based materials and applications

Journal of Applied Polymer ScienceVolume 79, Issue 7 p. 1324-1335 Antibacterial action of chitosan and carboxymethylated chitosan Xiao Fei Liu, Xiao Fei Liu Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of ChinaSearch for more papers by this authorYun Lin Guan, Corresponding Author Yun Lin Guan Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of ChinaResearch Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of China===Search for more papers by this authorDong Zhi Yang, Dong Zhi Yang Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of ChinaSearch for more papers by this authorZhi Li, Zhi Li Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of ChinaSearch for more papers by this authorKang De Yao, Kang De Yao Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of ChinaSearch for more papers by this author Xiao Fei Liu, Xiao Fei Liu Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of ChinaSearch for more papers by this authorYun Lin Guan, Corresponding Author Yun Lin Guan Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of ChinaResearch Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of China===Search for more papers by this authorDong Zhi Yang, Dong Zhi Yang Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of ChinaSearch for more papers by this authorZhi Li, Zhi Li Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of ChinaSearch for more papers by this authorKang De Yao, Kang De Yao Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, People's Republic of ChinaSearch for more papers by this author First published: 14 December 2000 https://doi.org/10.1002/1097-4628(20010214)79:7 3.0.CO;2-LCitations: 687Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract A series of chitosan with different molecular weights obtained by γ-irradiation depolymerization and another series of deacetylated chitosan were synthesized. Several N,O-carboxymethylated chitosan and O-carboxymethylated chitosan were also produced. The above samples were characterized by Fourier transform infrared spectroscopy (FTIR). Their antibacterial activities against E. coli were explored by the optical density method. The antibacterial activity of chitosan is influenced by its molecular weight, degree of deacetylation, concentration in solution, and pH of the medium. Antibacterial activities were also found to be increased in the order of N,O-carboxymethylated chitosan, chitosan, and O-carboxymethylated chitosan. Fluorescence of the FITC (fluorescein isothiocyanate)-labeled chitosan oligomers at the inside of the E. coli cell was observed by a confocal laser scanning microscope. The antibacterial activity of chitosan oligomers seems to be caused mainly by the inhibition of the transcription from DNA. © 2000 John Wiley & Sons, Inc. J Appl Polym Sci 79: 1324–1335, 2001 Citing Literature Volume79, Issue714 February 2001Pages 1324-1335 RelatedInformation