Produção Nacional
Revisado por pares
Aggregatibacter actinomycetemcomitans biofilm can be inactivated by methylene blue-mediated photodynamic therapy
2014; Elsevier BV; Volume: 12; Issue: 1 Linguagem: Inglês
10.1016/j.pdpdt.2014.10.002
ISSN1873-1597
AutoresLetícia Heineck Alvarenga, Renato Araújo Prates, Tania M. Yoshimura, Ilka Tiemy Kato, Luis C. Suzuki, Martha S. Ribeiro, Luis Rodolfo Ferreira, Sílvio Antônio dos Santos Pereira, Elizabeth Ferreira Martinez, Eduardo Saba‐Chujfi,
Tópico(s)Ocular Infections and Treatments
ResumoThe purpose of this study was to evaluate the antibacterial effects of photodynamic action of methylene blue (MB) against Aggregatibacter actinomycetemcomitans organized on biofilm. After the biofilm growth in 96 flat-bottom well plate, the following groups were used: control group, untreated by either laser or photosensitizer (PS); MB group or dark toxicity group, which was exposed to MB alone (100 μM) for 1 min (pre-irradiation time); laser group, irradiated with laser for 5 min in the absence of PS and three antimicrobial photodynamic inactivation (APDI) groups, with three exposure times of 1, 3 and 5 min of irradiation, corresponding to fluences of 15, 45, and 75 J/cm2 respectively. The results were compared to the control group for statistical proposes. Scanning electronic microscope analysis was used to access structural changes in biofilm. Red laser alone and MB alone were not able to inactivate bacterial biofilm. APDI groups showed differences when compared to the control group and they were dependent on the exposure time. No statistically significant differences were observed among the APDI groups at 1 and 3 min of irradiation. On the other hand, 5 min of APDI showed 99.85% of bacterial reduction (p = 0.0004). In addition, the biofilm loose its structure following 5 min APDI. The results of this study suggest that A. actinomycetemcomitans biofilm can be inactivated by MB mediated APDI.
Referência(s)