Portal de Periódicos da CAPES

A Simple Preparation of 5′-Biotinylated Oligonucleotides and Their use as Primers in Dideoxy-Sequencing of DNA

1991; Taylor & Francis; Volume: 10; Issue: 1-3 Linguagem: Inglês

10.1080/07328319108046491

2332-3892

Anthony J. Cocuzza, Robert J. Zagursky,

Advanced biosensing and bioanalysis techniques

Abstract 5′-Biotinylated oligonucleotides have proved valuable in a number of applications in molecular biology, particularly as hybridization probes and as primers for PCRI. This report describes a simple, efficient method for the preparation of 5′-biotinylated oligonucleotides and their use in a new application, as primers in an improved method for dideoxy sequencing of DNA.