Characterization of Cyprinus carpio brain nitric oxide synthase
1998; Elsevier BV; Volume: 242; Issue: 3 Linguagem: Inglês
10.1016/s0304-3940(98)00063-9
ISSN1872-7972
Autores Tópico(s)Renin-Angiotensin System Studies
ResumoNitric oxide synthase (NOS) activity is found both in soluble and in particulate fractions of the carp brain. The Km values for arginine are 2.8±0.5 and 3.3±0.4 μM for the soluble and particulate fractions, respectively. Ki for NG-monomethyl-l-arginine inhibitor are 2.6±0.5 and 2.9±0.6 μM, and activation energy for the breakdown of the substrate-enzyme complex 8120±710 and 4620±450 cal per mole. Carp enzyme shows higher affinity than rat NOS for Ca2+ and for the competitive inhibitor 7-nitroindazole.
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