Structural characterisation of a heteropolysaccharide by NMR spectra
2008; Elsevier BV; Volume: 112; Issue: 4 Linguagem: Inglês
10.1016/j.foodchem.2008.07.017
ISSN1873-7072
AutoresLibin Ye, Jingsong Zhang, Yan Yang, Shuai Zhou, Yanfang Liu, QingJui Tang, Xiuju Du, Hui Chen, Pan Yingjie,
Tópico(s)Polysaccharides Composition and Applications
ResumoAn undocumented water-soluble polysaccharide, LZ-C-1, was isolated from the fruiting bodies of the fungus, Ganoderma lucidum. The polysaccharide had a molecular weight of 7 × 103 Da, and was mainly composed of l-Fuc, d-Glc and d-Gal. LZ-C-1 had a sugar content of ∼96.4% as measured using the phenol–sulphuric acid method. As a precondition to understand the bioactivity, structural features of LZ-C-1 were investigated by a combination of total hydrolysis, methylation analysis, FT-IR and NMR studies. The results indicated that LZ-C-1 had a backbone of 1,6-disubstituted-α-galactopyranosyl, 1,2,6-trisubstituted-α-galactopyranosyl, 1,3-disubstituted-β-glucopyranosyl and 1,4,6-trisubstituted-β-glucopyranosyl residues. The branches were mainly composed of 1-substituted-β-glucopyranosyl and 1-substituted-α-fucopyranosyl residues.
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