Portal de Periódicos da CAPES

Incorporation and metabolism of cortisol in oocytes of tilapia (Oreochromis mossambicus)

2000; Wiley; Volume: 287; Issue: 7 Linguagem: Inglês

10.1002/1097-010x(20001201)287

1097-010X

Masatomo Tagawa, Keiko Suzuki, Jennifer L. Specker,

Reproductive biology and impacts on aquatic species

Journal of Experimental ZoologyVolume 287, Issue 7 p. 485-492 EndocrinologyFull Access Incorporation and metabolism of cortisol in oocytes of tilapia (Oreochromis mossambicus) Masatomo Tagawa, Corresponding Author Masatomo Tagawa [email protected] Department of Zoology, University of Rhode Island, Kingston, Rhode Island 02881Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan===Search for more papers by this authorKeiko Suzuki, Keiko Suzuki National Institute of Radiological Sciences, Chiba 263-8555, JapanSearch for more papers by this authorJennifer L. Specker, Jennifer L. Specker Department of Zoology, University of Rhode Island, Kingston, Rhode Island 02881 Graduate School of Oceanography, University of Rhode Island, Narragansett, Rhode Island 02882-1197Search for more papers by this author Masatomo Tagawa, Corresponding Author Masatomo Tagawa [email protected] Department of Zoology, University of Rhode Island, Kingston, Rhode Island 02881Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan===Search for more papers by this authorKeiko Suzuki, Keiko Suzuki National Institute of Radiological Sciences, Chiba 263-8555, JapanSearch for more papers by this authorJennifer L. Specker, Jennifer L. Specker Department of Zoology, University of Rhode Island, Kingston, Rhode Island 02881 Graduate School of Oceanography, University of Rhode Island, Narragansett, Rhode Island 02882-1197Search for more papers by this author First published: 06 November 2000 https://doi.org/10.1002/1097-010X(20001201)287:7 3.0.CO;2-6Citations: 18AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract The entry and metabolism of 3H-cortisol in oocytes were investigated using isolated follicles of the tilapia (Oreochromis mossambicus) in order to examine the mechanisms of incorporation of maternal hormones into oocytes. The composition of 3H-labeled steroids in the oocyte was analyzed by high-performance liquid chromatography. A significant amount of cortisol was converted to cortisone and an unidentified molecule by the follicular layer. The contents of 3H-cortisol and 3H-cortisone in the oocyte reached an equilibrium level within 12 hr, whereas the content of the unidentified metabolite continued to increase for 36 hr. The total content of the incorporated cortisol and its metabolites was proportional to cortisol in the medium over the concentration range of 5 ng/ml to 5 μg/ml. The amounts of cortisone and the unidentified molecule increased proportionally when the concentration of cortisol in the medium was lower than 500 ng/ml, whereas they reached a plateau when the concentration of cortisol exceeded 500 ng/ml. Cortisol entry was reversible, because 90% of cortisol and cortisone in the oocyte was lost within 18 hr when the medium was changed to that without 3H-cortisol. On the other hand, 50% of the unidentified molecule was preserved at the end of the incubation. In conclusion, the entry of cortisol into the oocyte was considered to be nonspecific and due probably to simple diffusion. However, a considerable amount of cortisol (50–70%) was specifically converted to cortisone and another unidentified molecule during passage through the follicular layer. J. Exp. Zool. 287:485–492, 2000. © 2000 Wiley-Liss, Inc. LITERATURE CITED Ayson FG, Kaneko T, Hasegawa S, Hirano T. 1994. Cortisol stimulates the size and number of mitochondrion-rich cells in the yolk-sac membrane of embryos and larvae of tilapia (Oreochromis mossambicus) in vitro and in vivo. J Exp Zool 272: 419–425. Brown CL, Bern HA. 1989. Thyroid hormones in early development, with special reference to teleost fishes. In: MP Schreibman, editors. Hormones and development, maturation, and senescence of neuroendocrine systems. New York: Academic Press. p 289–306. Hwang P, Wu S, Lin J, Wu L. 1992. Cortisol content of eggs and larvae of teleosts. Gen Comp Endocrinol 86: 189–196. de Jesus EG, Hirano T, Inui Y. 1991. Changes in cortisol and thyroid hormone concentrations during early development and metamorphosis in the Japanese flounder, Paralichthys olivaceus. Gen Comp Endocrinol 82: 369–376. Johanning KM, Specker JL. 1995. Characterization of yolk proteins during oocyte development of tilapia, Oreochromis mossambicus. Comp Biochem Physiol 112B: 177–189. Kishida M, Specker JL. 1993. Vitellogenin in tilapia (Oreochromis mossambicus): induction of two forms by estradiol, quantification in the plasma, and characterization in plasma and mucus. Fish Physiol Biochem 12: 171–182. Kobuke L, Specker JL, Bern HA. 1987. Thyroxine content in eggs and larvae of coho salmon, Onchorhynchus kisutch. J Exp Zool 242: 89–94. Schreck BS, Fitzpatrick MS, Feist GW, Yeoh C-G. 1991. Steroids: developmental continuum between mother and offspring. In: Scott AP, Sumpter JP, Kime DE, Rolfe MS, editors. Proceedings of the 4th International Symposium on the Reproductive Physiology of Fish, Sheffield. FishSymp 91:256–258. Scott AP, Vermeirssen ELM. 1994. Production of conjugated steroids by teleost gonads and their role as pheromones. In: KG Davey, editors. Perspectives in comparative endocrinology. Ottawa: National Research Council. p 645–654. Specker JL, Sullivan CV. 1994. Vitellogenesis in fishes: status and perspectives. In: KG Davey, editors. Perspectives in comparative endocrinology. Ottawa: National Research Council. p 304–315. Stratholt ML, Donaldson EM, Liley NR. 1997. Stress-induced elevation of plasma cortisol in adult female coho salmon (Oncorhynchus kisutch) is reflected in egg cortisol content but does not appear to affect early development. Aquaculture 158: 141–153. Tagawa M. 1996. Current understanding of the presence of hormones in fish eggs. In: Y Watanabe, editors. Survival strategies in early life stages of marine resources. Rotterdam: A.A. Balkema. p 27–38. Tagawa M, Tanaka M, Matsumoto S, Hirano T. 1990. Thyroid hormones in eggs of various freshwater, marine and diadromous teleosts and their changes during egg development. Fish Physiol Biochem 8: 515–520. Tagawa M, Ogasawara T, Sakamoto T, Miura T, Yamauchi K, Hirano T. 1994. Thyroid hormone concentrations in the gonad of wild chum salmon during maturation. Fish Physiol Biochem 13: 233–240. Tetsuka M, Milne M, Simpson GE, Hillier SG. 1999. Expression of 11β-hydroxysteroid dehydrogenase, glucocorticoid receptor, and mineralocorticoid recepter genes in rat ovary. Biol Reprod 60: 330–335. Weber GM, Okimoto DK, Richman III NH, Grau EG. 1992. Patterns of thyroxine and triiodothyronine in serum and follicle-bound oocytes of the tilapia, Oreochromis mossambicus, during oogenesis. Gen Comp Endocrinol 85: 392–404. Yeoh CG, Schreck CB, Feist GW, Fitzpatrick MS. 1994. Endogenus steroid metabolism is indicated by fluctuations of endogenous steroid and steroid glucuronide levels in early development of the steel head trout (Onchrhynchus mykiss). Gen Comp Endocrinol 103: 107–114. Citing Literature Volume287, Issue71 December 2000Pages 485-492 ReferencesRelatedInformation