Hydrolysis of plant polysaccharides and GLC analysis of their constituent neutral sugars
1979; Elsevier BV; Volume: 18; Issue: 3 Linguagem: Inglês
10.1016/s0031-9422(00)81879-8
ISSN1873-3700
AutoresA. T. Mankarios, Christopher F. Jones, Michael C. Jarvis, David R. Threfall, John Friend,
Tópico(s)Biofuel production and bioconversion
ResumoThe release and degradation of sugars from onion cell walls and potato cell wall polysaccharides were followed during hydrolysis with trifluoroacetic acid so that the optimum hydrolysis conditions could be determined. After 6 hr hydrolysis in 2 M acid at 100°, calculated recovery factors of different monosaccharides from potato pectic fractions ranged from 61 to 96%. Lower yields of monosaccharides were obtained from intact onion cell walls, while the yield from cellulose was less than 0.2%. A new GLC column for the separation of alditol acetates derived from cell wall sugars is described.
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