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Sulforaphane, Erucin, and Iberin Up-Regulate Thioredoxin Reductase 1 Expression in Human MCF-7 Cells

2005; American Chemical Society; Volume: 53; Issue: 5 Linguagem: Inglês

10.1021/jf048153j

1520-5118

Wei Wang, Shuran Wang, A. Forbes Howie, Geoffrey J. Beckett, Richard Mithen, Yongping Bao,

Glutathione Transferases and Polymorphisms

Isothiocyanates (ITCs) found in cruciferous vegetables are potentially important anticarcinogenic phytochemicals for many types of cancers including breast cancer. In this study, we have shown that three isothiocyanates, sulforaphane, erucin, and iberin, are potent inducers of thioredoxin reductase 1 (TrxR1) in human breast cancer MCF-7 cells. Sulforaphane, erucin, and iberin at 1 μM induce TrxR1 mRNA 2−3-fold within 8 h of treatment, and induce mRNA 5−7-fold with 12 μM ITC treatments. Selenium did not affect sulforaphane-induced TrxR1 mRNA levels, but significantly enhanced both TrxR1 protein expression (up to 9-fold in erucin treatment) and corresponding activities. These results suggest that dietary ITCs are important factors in the regulation of redox status through the induction of the selenoprotein thioredoxin reductase. Keywords: Isothiocyanates; sulforaphane; erucin; iberin; selenium; thioredoxin reductase