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Neuronal medium that supports basic synaptic functions and activity of human neurons in vitro

2015; National Academy of Sciences; Volume: 112; Issue: 20 Linguagem: Inglês

10.1073/pnas.1504393112

1091-6490

Cédric Bardy, Mark van den Hurk, Tameji Eames, Cynthia Marchand, Rubén Hernández, M Kellogg, Mark A.J. Gorris, Ben Galet, Vanessa Palomares, J. Elliott Brown, Anne G. Bang, Jérôme Mertens, Lena Böhnke, Leah Boyer, Suzanne Simon, Fred H. Gage,

Anesthesia and Neurotoxicity Research

Significance Neuronal cultures are very valuable to learn about basic principles of the nervous system. In vivo, neural electrical activity is the essence of nervous system function, controlling emotion, memory, sensory modalities, and behavior. In this study, we discovered that many crucial neurophysiological properties were strongly altered in classic culture media that are widely used by the research community. To overcome this problem, we designed and tested a new tissue culture neuromedium that adequately supports in vitro neuronal activity. The improvements made in this medium reduce the gap between in vivo brain physiological conditions and neuronal models in vitro. Improving physiological conditions in vitro may lead to more successful translation from bench to clinics.