In-frame TCR δ gene rearrangements play a critical role in the αβ/γδ T cell lineage decision
1995; Cell Press; Volume: 2; Issue: 6 Linguagem: Inglês
10.1016/1074-7613(95)90006-3
ISSN1097-4180
AutoresFerenc Livák, Howard T. Petrie, I. Nicholas Crisps, David G. Schatz,
Tópico(s)CAR-T cell therapy research
ResumoUsing a quantitative multiprobe Southern blot analysis, we demonstrate the surprising result that a significant proportion of alpha beta T cells and thymocytes retain T cell receptor delta locus sequences. A substantial portion of the retained delta locus is in a fully V-to-D-to-J rearranged configuration and 20% of these delta rearrangements are functional, significantly less than the 33% predicted for random gene rearrangements. Our observations are in conflict with the idea that alpha beta and gamma delta T cells derive from distinct precursors and suggest that commitment of a common precursor to the gamma delta lineage depends upon expression of a gamma delta T cell receptor. We propose that the intrathymic T cell lineage decision is determined by a competition between the production of functional gamma delta and beta-pre-T cell receptor complexes.
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