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Determination of chlorophyll pigments in crude and degummed canola oils by HPLC and spectrophotometry

1989; Wiley; Volume: 66; Issue: 8 Linguagem: Inglês

10.1007/bf02670098

1558-9331

J. K. Daun, C. T. Thorsteinson,

Advanced Nanomaterials in Catalysis

Abstract Chlorophyll pigments in crude and degummed canola oils were analyzed by spectrophotometry using a modified AOCS Method and by reversed phase HPLC. HPLC showed that crude canola oils contained very little chlorophyll a or b , these pigments having been converted to pheophytins and other pigments with similar spectral properties. The ratio of chlorophyll a∶b in the seed was found to be about 3∶1 while the ratio of pheophytin a∶b in the oil was about 9∶1. As the AOCS Method for determining oil chlorophyll was calibrated for pure chlorophyll, the use of this method on crude canola oil results in a significant error. Recalibration of the spectrophotometric procedure with pheophytin gave better agreement with the HPLC method.