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Constitutive and LPS-induced gene expression in a macrophage-like cell line from the rainbow trout (Oncorhynchus mykiss)

2000; Elsevier BV; Volume: 24; Issue: 6-7 Linguagem: Inglês

10.1016/s0145-305x(00)00019-7

1879-0089

John L. Brubacher, Christopher J. Secombes, Jun Zou, Niels C. Bols,

Immune Response and Inflammation

The lack of cell lines from mononuclear phagocytes of salmonid fish has impeded the study of immune function at a cellular level in these economically and ecologically important animals. Here, we report on the further characterization of RTS11, a previously described macrophage-like cell line from the rainbow trout spleen, with regard to its expression of a number of immunologically relevant genes. Analysis of gene expression by reverse transcription-polymerase chain reaction, using rainbow-trout-specific primers demonstrated that RTS11 cells express the β chain of the class II major histocompatibility complex, the cytokines transforming growth factor-β (TGF-β) and interleukin-1β (IL-1β), and cyclo-oxygenase-2 (COX-2). Inducing the cells with lipopolysaccharide led to increased expression of IL-1β and COX-2, as determined by Northern blotting. These results together suggest that RTS11 retains many of the characteristics expected of mature macrophages, and should be a valuable tool for further study of the expression and function of these immunomodulatory proteins in fish.