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Label-free DNA sequence detection using oligonucleotide functionalized optical fiber

2006; American Institute of Physics; Volume: 89; Issue: 16 Linguagem: Inglês

10.1063/1.2364459

1520-8842

Xingwei Wang, Kristie L. Cooper, Anbo Wang, Juncheng Xu, Zhuang Wang, Yan Zhang, Zhijian Tu,

Nanofabrication and Lithography Techniques

The authors present a label-free method for direct detection of deoxyribonucleic acid (DNA) sequences. The capture DNA is immobilized onto the surface of a silica optical fiber tip by means of the layer-by-layer electrostatic self-assembly technique. Hybridization of target DNA with complementary capture DNA increases the optical thickness of the fiber tip. This phenomenon can be detected by demodulation of the spectrum of a Fabry-Pérot cavity fabricated in the optical fiber. Experimental results demonstrate sequence specificity and sensitivity to nanogram quantities of target DNA sequences with short (∼5min) hybridization time.