Functions of coenzyme Q 10 in inflammation and gene expression
2008; Wiley; Volume: 32; Issue: 1-4 Linguagem: Inglês
10.1002/biof.5520320121
ISSN1872-8081
AutoresConstance Schmelzer, Inka Lindner, Gerald Rimbach, Petra Niklowitz, Thomas Menke, Frank Döring,
Tópico(s)Free Radicals and Antioxidants
ResumoAbstract Clinical studies demonstrated the efficacy of Coenzyme Q 10 (CoQ 10 ) as an adjuvant therapeutic in cardiovascular diseases, mitochondrial myopathies and neurodegenerative diseases. More recently, expression profiling revealed that Coenzyme Q 10 (CoQ 10 ) influences the expression of several hundred genes. To unravel the functional connections of these genes, we performed a text mining approach using the Genomatix BiblioSphere. We identified signalling pathways of G‐protein coupled receptors, JAK/STAT, and Integrin which contain a number of CoQ 10 sensitive genes. Further analysis suggested that IL5, thrombin, vitronectin, vitronectin receptor, and C‐reactive protein are regulated by CoQ 10 via the transcription factor NFκB1. To test this hypothesis, we studied the effect of CoQ 10 on the NFκB1‐dependent pro‐inflammatory cytokine TNF‐α. As a model, we utilized the murine macrophage cell lines RAW264.7 transfected with human apolipoprotein E3 (apoE3, control) or pro‐inflammatory apoE4. In the presence of 2.5 μM or 75 μM CoQ 10 the LPS‐induced TNF‐α response was significantly reduced to 73.3±2.8% and 74.7±8.9% in apoE3 or apoE4 cells, respectively. Therefore, the in silico analysis as well as the cell culture experiments suggested that CoQ 10 exerts anti‐inflammatory properties via NFκB1‐dependent gene expression.
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