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Lamellipodial Versus Filopodial Mode of the Actin Nanomachinery

2004; Cell Press; Volume: 118; Issue: 3 Linguagem: Inglês

10.1016/j.cell.2004.07.019

1097-4172

Marisan Mejillano, Shin‐ichiro Kojima, Derek A. Applewhite, Frank B. Gertler, Tatyana Svitkina, Gary G. Borisy,

Microtubule and mitosis dynamics

Understanding how a particular cell type expresses the lamellipodial or filopodial form of the actin machinery is essential to understanding a cell's functional interactions. To determine how a cell "chooses" among these alternative modes of "molecular hardware," we tested the role of key proteins that affect actin filament barbed ends. Depletion of capping protein (CP) by short hairpin RNA (shRNA) caused loss of lamellipodia and explosive formation of filopodia. The knockdown phenotype was rescued by a CP mutant refractory to shRNA, but not by another barbed-end capper, gelsolin, demonstrating that the phenotype was specific for CP. In Ena/VASP deficient cells, CP depletion resulted in ruffling instead of filopodia. We propose a model for selection of lamellipodial versus filopodial organization in which CP is a negative regulator of filopodia formation and Ena/VASP has recruiting/activating functions downstream of actin filament elongation in addition to its previously suggested anticapping and antibranching activities.