Portal de Periódicos da CAPES

Isolation and analysis of candidate myeloid tumor suppressor genes from a commonly deleted segment of 7q22

2005; Elsevier BV; Volume: 85; Issue: 5 Linguagem: Inglês

10.1016/j.ygeno.2005.01.013

1089-8646

Nicole P. Curtiss, Jeannette M. Bonifas, Jennifer Lauchle, Jason D. Balkman, Christian P. Kratz, Brooke M. Emerling, Eric D. Green, Michelle M. Le Beau, Kevin Shannon,

Acute Myeloid Leukemia Research

Monosomy 7 and deletions of 7q are recurring leukemia-associated cytogenetic abnormalities that correlate with adverse outcomes in children and adults. We describe a 2.52-Mb genomic DNA contig that spans a commonly deleted segment of chromosome band 7q22 identified in myeloid malignancies. This interval currently includes 14 genes, 19 predicted genes, and 5 predicted pseudogenes. We have extensively characterized the FBXL13, NAPE-PLD, and SVH genes as candidate myeloid tumor suppressors. FBXL13 encodes a novel F-box protein, SVHis a member of a gene family that contains Armadillo-like repeats, and NAPE-PLD encodes a phospholipase D-type phosphodiesterase. Analysis of a panel of leukemia specimens with monosomy 7 did not reveal mutations in these or in the candidate genes LRRC17, PRO1598, and SRPK2. This fully sequenced and annotated contig provides a resource for candidate myeloid tumor suppressor gene discovery.