Prostaglandin production by horse embryos and the effect of co-culture of embryos with endometrium from pregnant mares
1989; Bioscientifica; Volume: 87; Issue: 1 Linguagem: Inglês
10.1530/jrf.0.0870331
ISSN1741-7899
AutoresE.D. Watson, Patricia L. Sertich,
Tópico(s)Reproductive System and Pregnancy
ResumoSummary. Embryos, endometrial biopsies, and uterine lavage fluid were collected from pregnant and non-pregnant mares 14 days after ovulation. Embryos were cultured for 20·5 h with and without endometrial tissue from pregnant mares, and endometrial tissue was cultured alone. Endometrial content of PGF tended to be higher (P = 0·06) in non-pregnant than in pregnant mares, but the amount of PGF released from tissue during culture was similar for pregnant and non-pregnant mares. Lavage fluid from non-pregnant mares also tended (P = 0·08) to contain higher concentrations of PGF. Co-incubation of embryos with endometrium from pregnant mares significantly (P = 0·01) lowered concentrations of PGF in medium. Tissue concentrations and release of PGE-2 and 6-keto-PGF-1α were similar in endometrial samples from pregnant and non-pregnant mares and prostaglandin production was unaffected by the presence of an embryo during incubation. Horse embryos released all three prostaglandins during a 20·5-h incubation. Keywords: mare; prostaglandins; pregnancy; embryo
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