Enantioselective esterification reaction using immobilized Candida rugosa lipase on poly( N ‐vinyl‐2‐pyrrolidone‐ co ‐styrene) hydrogel
2004; Wiley; Volume: 92; Issue: 5 Linguagem: Inglês
10.1002/app.20342
ISSN1097-4628
AutoresAiza Harun, Mahiran Basri, M. B. Ahmad, Abu Bakar Salleh,
Tópico(s)Analytical Chemistry and Chromatography
ResumoAbstract Lipase from Candida rugosa was immobilized on poly( N ‐vinyl‐2‐pyrrolidone‐ co ‐styrene) hydrogel (poly‐(VP‐ co ‐ST)) with ethylene dimethacrylate and α,α'‐azoisobutyronitrile, which act as crosslinker and initiator, respectively. Three different compositions of monomers were used, namely VP(%):ST(%), 10:90, 50:50, and 70:30 (wt(%)/wt(%)). The immobilized lipases were used in the enantioselective esterification of ( R , S )‐2‐(4‐chlorophenoxy)‐propanoic acid with n ‐tetradecanol. The optimum reaction condition of the enantioselective esterification for the native lipase and the poly(VP‐ co ‐ST) hydrogel immobilized lipases was determined with respect to temperature, solvents, and initial water activity ( a w ). The optimum temperature obtained was 40°C, with the poly(VP‐ co ‐ST) hydrogel immobilized lipase VP(%)/ST(%):10:90 showing the highest enantiomeric excess. In the solvent effect studies, the best solvents for high enantioselectivity were chloroform and carbon tetrachloride. In the a w studies, optimum α w for NL, VP(%):ST(%), 10:90, and 50:50 was 0.328, while for VP(%):ST(%), 70:30, it was 0.55. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 92: 3381–3386, 2004
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