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Determination of TBA number by high performance liquid chromatography

1981; Wiley; Volume: 58; Issue: 7 Linguagem: Inglês

10.1007/bf02887320

1558-9331

Yukio Kakuda, D.W. Stanley, F.R. van de Voort,

Animal Nutrition and Physiology

Abstract A high performance liquid chromatographic (HPLC) method for the quantitation of malonaldehyde in aqueous distillates was developed. Compared with the standard TBA test, the HPLC method was faster, and less affected by side feactions. A total of 5 min was necessary to assay each distillate and only malonaldehyde was detected. The standard curves were reproducible and standards were stable for up to 6 days. The HPLC method could detect malonaldehyde levels ranging from 1×10 −11 to 4×10 −11 mol/10 µ L and either peak height or peak area could be used to quantitate the malonaldehyde concentration. The coefficient of determination between absorbance values determined by the TBA test and peak heights determined by HPLC was 0.946. Twenty‐one freeze‐dried chicken samples with TBA numbers ranging from 3.93 to 16.6 were used for this correlation.