Modulation of soluble and membrane-bound TNF-induced phenotypic and functional changes of human brain microvascular endothelial cells by recombinant TNF binding protein I
1997; Elsevier BV; Volume: 77; Issue: 1 Linguagem: Inglês
10.1016/s0165-5728(97)00067-2
ISSN1872-8421
AutoresJ. Lou, A. Ythier, Danielle Burger, Lei Zheng, Pierre Juillard, Rudolf Lucas, Jean‐Michel Dayer, Georges E. Grau,
Tópico(s)S100 Proteins and Annexins
ResumoIn this study, the effects of TNF binding protein I (TBP I) on TNF-induced changes of human brain microvascular endothelial cells (MVEC) were investigated. TBP I completely abolished TNF-induced IL-6 production and E-selectin induction, while it partially inhibited TNF-induced IL-8 production and up-regulation of ICAM-1 and VCAM-1. Moreover, TBP I significantly inhibited TNF-induced cytotoxicity and leukocyte adherence on human brain MVEC. The inhibitory activity of TBP I for TNF was dose-dependent and related to the time of administration after TNF stimulation. In addition, TBP I inhibited membrane-bound TNF induced activation of human brain MVEC, but the concentration required was about 10-fold higher than that for soluble TNF. These results indicate a therapeutic potential for TBP I in diseases of the central nervous system associated with TNF overproduction.
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