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Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray

2014; Landes Bioscience; Volume: 9; Issue: 6 Linguagem: Inglês

10.4161/epi.28790

1559-2308

Sebastián Morán, Miguel Vizoso, Anna Martínez‐Cardús, Antonio Gómez, Xavier Matías‐Guiu, Sebastián Chiavenna, Andrés G Fernandez, Manel Esteller,

RNA modifications and cancer

A formalin-fixed paraffin-embedded (FFPE) sample usually yields highly degraded DNA, which limits the use of techniques requiring high-quality DNA, such as Infinium Methylation microarrays. To overcome this restriction, we have applied an FFPE restoration procedure consisting of DNA repair and ligation processes in a set of paired fresh-frozen (FF) and FFPE samples. We validated the FFPE results in comparison with matched FF samples, enabling us to use FFPE samples on the Infinium HumanMethylation450 Methylation array.