Immunocytochemical localization of μ-opioid receptor in the rat caudate-putamen
1995; Elsevier BV; Volume: 184; Issue: 3 Linguagem: Inglês
10.1016/0304-3940(94)11192-l
ISSN1872-7972
AutoresTakeshi Kaneko, Masabumi Minami, Masamichi Satoh, Nohoru Mizuno,
Tópico(s)Pharmacological Receptor Mechanisms and Effects
ResumoA guinea pig antibody against a C-terminal peptide of rat μ-opioid receptor (MOR) was produced to examine the distribution of MOR in the rat caudate-putamen (CP). The anti-peptide antibody recognized a protein of Mr 69 000 in Triton X-100 extract of rat brain and in the membrane fraction of MOR-expressing culture cells. Intense MOR-like immunoreactivity (LI) was observed in island-like areas of the CP. Some MOR-LI was located on the cell bodies and dendrites of CP neurons. Double immunofluorescence study revealed that the intensely MOR-immunoreactive areas showed weak calbindin-LI, surrounded by intensely calbindin-positive regions. The results indicate that MOR-LI is enriched in the 'patches' of the neostriatal mosaic compartmentation.
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